The effect of secretory leukocyte protease inhibitor amnion membrane on incisional wound healing

Abstract

In human body, biomaterials can be used to accelerate the wound healing process. Amniotic membrane-derived secretory leukocyte protease inhibitor (SLPI) is one of the biomaterials which able to accelerate the wound healing process by its several functions such as protease inhibition, leukocytes activity control, growth factor regulation such as transforming growth factor β (TGF-β), and its anti inflammatory, anti bacterial also anti retroviral properties. Objectives: To analyzed the SLPI effect on the TNF-α expression and the quantity of new blood vessel in the incisional wound healing. SLPI are produced from Essericea coli TOP10 as the cloning host, BL21 (DE3) strains as the expression host and pET30a plasmids were used for the expression system construction. The incision wound was made in dorsal skin area, then treated with SLPI dose 0.03 cc, 0.45 cc, 0.06 cc. After treatment, the wound covered with hypafix. The control group only covered with hypafix. In the next four days, the dorsal skin area is biopsied, then the quantity of TNF-α expression and blood vessels was analyzed with hematoxylin eosin and immunohistochemistry staining. The SLPI 0.045 cc showed the higher quantity of new blood vessel compared the SLPI 0.03 cc and control group (p < 0.05). While the TNF-α expression in the SLPI 0.045 cc is lower than SLPI 0.03 cc (p = 0.002) and control group (p = 0.000). The SLPI capable to accelerate the wound healing by decreasing the TNF-α expression and increased the new blood vessel quantity at 0.045 cc dose.

Keywords

• Amniotic membrane
• Blood vessel
• Secretory leukocyte protease inhibitor
• TNF-α expression
• human&health