Development of novel ELISA based on tyramine signal amplification for sensitive detection of danofloxacin

Abstract

ObjectiveTo develop a novel ELISA for sensitive and naked-eye detection of danofloxacin （DAN）.MethodsBased on TYR signal amplification， a novel ELISA was developed for sensitive detection of DAN. Analytical performance of the method was conducted by sensitivity and specificity experiments. The method was used for detecting DAN-spiked chicken and pork samples to verify.ResultsThis method could be used to qualitatively detect DAN with naked eyes at a cut-off value of 0.4 ng/mL， which was 25-fold lower than that of the traditional ELISA （10 ng/mL）. The method exhibited no cross-reaction of DAN with six other drugs in buffer. The method was successfully used for detecting DAN-spiked chicken and pork samples.ConclusionThe developed novel ELISA exhibits high sensitivity and specificity， and can be used for the danofloxacin detection in actual samples by naked eye.

Keywords

• tyramine signal amplification
• aunps
• ascorbic acid
• danofloxacin