A Fluorescent Sensor-Assisted Paper-Based Competitive Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ampicillin in Hospital Wastewater
Honggui Lin,
Feixiang Fang,
Jiahui Zang,
Jianlong Su,
Qingyuan Tian,
Ranjith Kumar Kankala,
Xuexia Lin
Affiliations
Honggui Lin
Fujian Province Key Laboratory of Ship and Ocean Engineering, Marine Engineering College, Jimei University, Xiamen 361021, China
Feixiang Fang
Department of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Jiahui Zang
Department of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Jianlong Su
Department of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Qingyuan Tian
Fujian Province Key Laboratory of Ship and Ocean Engineering, Marine Engineering College, Jimei University, Xiamen 361021, China
Ranjith Kumar Kankala
Department of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Xuexia Lin
Department of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
In this study, a convenient assay method has been developed based on labeled functional nucleic acids (H-DNA) and a competitive fluorescent lateral flow immunoassay (CF-LFI) for ampicillin (AMP) detection. Herein, we designed the tunable AMP probes for AMP detection based on the AMP aptamer, and the secondary DNA fragment. The probes can generate tunable signals on the test line (T line) and control line (C line) according to the concentration of AMP. The accuracy of detection was improved by optimizing the tunable AMP probes. Under the optimal conditions, the linear concentration of AMP detection is ranged from 10 to 200 ng/L with a limit of quantitation (LOQ) value of 2.71 ng/L, and the recovery is higher than 80.5 %. Moreover, the developed method shows the potential application for AMP detection in the hospital wastewater.
