Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

P. Guichard; V. Hamel; M. Le Guennec; N. Banterle; I. Iacovache; V. Nemčíková; I. Flückiger; K. N. Goldie; H. Stahlberg; D. Lévy; B. Zuber; P. Gönczy

doi:10.1038/ncomms14813

Nature Communications (Mar 2017)

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

P. Guichard,
V. Hamel,
M. Le Guennec,
N. Banterle,
I. Iacovache,
V. Nemčíková,
I. Flückiger,
K. N. Goldie,
H. Stahlberg,
D. Lévy,
B. Zuber,
P. Gönczy

Affiliations

P. Guichard: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
V. Hamel: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
M. Le Guennec: Department of Cell Biology, Sciences III, University of Geneva
N. Banterle: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
I. Iacovache: Institute of Anatomy, University of Bern
V. Nemčíková: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
I. Flückiger: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
K. N. Goldie: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel
H. Stahlberg: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel
D. Lévy: Institut Curie, PSL Research University, UMR 168, Centre de Recherche, 26 rue d’ULM, Paris 75231, France
B. Zuber: Institute of Anatomy, University of Bern
P. Gönczy: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)

DOI: https://doi.org/10.1038/ncomms14813
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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The centriole is an organelle composed of rings of SAS-6 proteins that form a cartwheel structure. Here the authors develop a cell-free system to examine core cartwheel assembly ofC. reinhardtiiproteins and discover that CrSAS-6 has autonomous properties that facilitates self-organized stacking of pairs of rings.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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