Detection of mecA and staphylococcal cassette chromosome mec gene isolated from Northeast Part of Karnataka Staphylococcus aureus isolates

Abstract

Background: Methicillin- resistant Staphylococcus aureus (MRSA) strains are predominantly significant because they are leading healthcare- associated infections worldwide and even majorly emerged as community associated infections. Methods: A total of 40 S. aureus isolates were collected from Navodaya Institute of Medical Sciences, Raichur Institute of Medical Sciences, and Mid Tech Diagnostic Laboratory, Raichur, and analyzed for mecA, Panton–Valentine leukocidin (PVL), and staphylococcal cassette chromosome mec (SCCmec) typing by gradient and Multiplex polymerase chain reaction (PCR). Results: Out of 40 S. aureus isolates, 7 (17.8%) were resistant and 33 (82.8%) were sensitive for methicillin antibiotic. The seven isolates showed positive for Vancomycin /cefoxitin Ezy MIC strip and oxacillin antibiotic MIC was ≥ 8μg/ml respectively PCR detection has revealed, seven isolates were positive for mecA and SCCmec typing, but none of the isolates has shown positive for PVL genes. The study shows the presence of SCCmec Type II and III was confirmed by multiplex PCR in hospital-acquired MRSA isolate and mecA gene positive of seven strains of MRSA Conclusion: The study demonstrated high specificity and positive prediction of the detection of MRSA from clinical samples.

Keywords

• hospital acquired/community acquired methicillin-resistant staphylococcus aureus
• meca
• mrsa
• pvl
• staphylococcus aureus
• staphylococcal cassette chromosome mec