E3S Web of Conferences (Jan 2023)

Characterization of biosurfactants produced by the Bacillus amyloliquefaciens ВКМА В-12464

  • Osmorskaya Zlata,
  • Ezhkin Nikita,
  • Gordeev Alexander,
  • Biktasheva Liliya,
  • Selivanovskaya Svetlana

DOI
https://doi.org/10.1051/e3sconf/202339201013
Journal volume & issue
Vol. 392
p. 01013

Abstract

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Biosurfactants are amphiphilic substances with a wide range of uses. Biosurfactants are being researched in various areas of biotechnology. They are especially widely used in the development of new environmentally friendly methods for increasing oil production, as well as in agriculture as an effective biocontrol agent. However, the possibility of finding strains producing biosurfactants and the efficiency of their production is a separate task for research. The aim of this work was to evaluate the dynamics of biosurfactant production by the Bacillus amyloliquefaciens VKMA B-12464 strain, as well as to evaluate its chemical nature. During the research, the resulting biosurfactant was characterized by TLC and IR spectroscopy. It has been established that the biosurfactant produced by Bacillus amyloliquefaciens belongs to lipopeptides, and its highest yield is observed on the 7th day of cultivation.