Validation of an Analytical Method for the Simultaneous Determination of Hyaluronic Acid Concentration and Molecular Weight by Size-Exclusion Chromatography
Luis Antonio Suárez-Hernández,
Rosa María Camacho-Ruíz,
Enrique Arriola-Guevara,
Eduardo Padilla-Camberos,
Manuel Reinhart Kirchmayr,
Rosa Isela Corona-González,
Guadalupe María Guatemala-Morales
Affiliations
Luis Antonio Suárez-Hernández
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Normalistas 800, Guadalajara C.P. 44270, Jalisco, Mexico
Rosa María Camacho-Ruíz
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Camino Arenero 1227, Zapopan C.P. 45019, Jalisco, Mexico
Enrique Arriola-Guevara
Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, Guadalajara C.P. 44430, Jalisco, Mexico
Eduardo Padilla-Camberos
Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Normalistas 800, Guadalajara C.P. 44270, Jalisco, Mexico
Manuel Reinhart Kirchmayr
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Camino Arenero 1227, Zapopan C.P. 45019, Jalisco, Mexico
Rosa Isela Corona-González
Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, Guadalajara C.P. 44430, Jalisco, Mexico
Guadalupe María Guatemala-Morales
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Normalistas 800, Guadalajara C.P. 44270, Jalisco, Mexico
The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and pharmaceutical applications; thus, it is important to have validated, analytical methods to ensure confidence and security of its use (and to save time and resources). In this work, a size-exclusion chromatography method (HPLC-SEC) was validated to determine the concentration and molecular distribution of HA simultaneously. Analytical curves were developed for concentration and molecular weight in the ranges of 100–1000 mg/L and 0.011–2.200 MDa, respectively. The HPLC-SEC method showed repeatability and reproducibility greater than 98% and limits of detection and quantification of 12 and 42 mg/L, respectively, and was successfully applied to the analysis of HA from a bacterial culture, as well as cosmetic, and pharmaceutical products.
