Journal of Experimental Pharmacology (May 2025)
Influence of the Solvent and the Harvesting Site on the Content of Phenolic Compounds and the in Vitro Antidiabetic Potential of Leafy Stems and Roots of Phyllanthus amarus Schum. and Thonn.
Abstract
Abdoul Aziz Zanté,1 Daouda Zoungo,1 Yacouba Sanou,1 Pawendé Kabré,1 Relwendé Justin Ouédraogo,2 Lazare Belemnaba,2 Lassina Ouattara,1 Paulin Ouoba,1 Georges Anicet Ouédraogo1 1Ecole Doctorale Sciences Naturelles et Agronomie, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso; 2Département de la Médecine Traditionnelle, de la Pharmacopée et de la Pharmacie, Institut de Recherche en Sciences de la Santé, Ouagadougou, Burkina FasoCorrespondence: Abdoul Aziz Zanté, Ecole Doctorale Sciences Naturelles et Agronomie, Université Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Bobo-Dioulasso, Burkina Faso, Tel +22676588716, Email [email protected]: The objective was to verify the impact of the solvent and the harvesting site on the content of phenolic compounds as well as the in vitro antidiabetic activity of leafy stems and roots of Phyllanthus amarus.Methods: The polyphenols and total flavonoids were measured on the crude extracts, obtained after maceration for 48 hours with acetone-water 50:50 (v/v), and ethanol-water 70:30 (v/v) . These extracts were evaluated for their antioxidant properties by DPPH, ABTS, and iron reduction (FRAP) tests. Finally, the α-amylase inhibitory activity of the crude extracts was determined by the method using DNS.Results: The results show that acetone-water favors polyphenol extraction, with a maximum content of 32.62 ± 0.85 mg EAG/100 mg DE in leafy stems from Banfora (LSBaAw). In contrast, ethanol-water extracted more flavonoids, with 4.59 ± 0.02 mg EQ/100 mg DE in roots from Bobo (RBoEw). For antioxidant activity, the ethanol-water extract of Bobo leafy stems (LSBoEw) showed the highest ABTS free radical scavenging activity (81.34 ± 1.07 μg/mL). In comparison, the ethanol-water extract of Banfora roots (RBaEw) showed the best DPPH free radical scavenging activity (55.71 ± 2.48 μg/mL). On the other hand, the acetone-water extract of Banfora leafy stems (LSBaAw) showed the highest iron reduction activity (15,445.81 ± 835.75 μmol EAA/100 mg DE). Finally, the highest α-amylase inhibitory activity was observed with ethanol-water extracts from roots (RBaEw: 98.45 ± 0.38%; RBoEw: 96.56± 0.31%).Conclusion: These results underline the importance of the choice of solvent, organ, and harvesting site in optimizing the use of Phyllanthus amarus. Further studies involving other solvents and environmental conditions will enable us to refine these observations and optimize the pharmacological potential of this plant.Keywords: Phyllanthus amarus, root, leafy stem, solvent, site
