Environmental benign analytical quality by design and UPLC method development for Betamethasone and Calcipotriene in ointment

Abstract

The present study aimed to estimate Betamethasone and Calcipotriene in bulk and ointment formulation using a validated UPLC method by applying analytical quality design with green analytical chemistry principles. The optimal method resulted with Dikma Endeversil C18 ODS (2.1 × 50 mm, 1.8 µm) column at a UV detection of 254 nm encompassed ethanol and potassium dihydrogen phosphate (3.0 pH) buffer 51:49 (v/v), with a flow rate of 0.31 mL/min. The detector response was linear at 125–750 and 12.5–75 µg/mL for Betamethasone and Calcipotriene with a detection limit and quantification of 12.484, 37.831 and 3.229, 9.785 µg/mL, respectively. The % recovery was found to be within limits of less than 1.5%. Overall, the AQbD based developed method was greener and confirmed by the greenness evaluation tools. Hence, the optimized technique is environment friendly, simple, robust for the concurrent assessment of Betamethasone and Calcipotriene in bulk and ointment formulation.

Keywords

• betamethasone
• calcipotriene
• green analytical chemistry
• rotatable central composite design
• uplc