The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance

Abstract

Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava), Zea mays (maize), Oryza sativa (rice), Triticum aestivum (wheat), Sorghum bicolor (sorghum), Brassica rapa (rape seed), Solanum tuberosum (potato), Solanum lycopersicum (tomato), Elaes guineensis (oil palm), Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs.

Keywords

• plant
• transformation
• plasmid
• prap15
• prap17 overexpression
• heterologous expression
• ectopic expression
• rna interference (rnai)
• crop
• genetic
• engineering
• nematode
• bri1-associated receptor kinase 1
• bak1