Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
Van Luu,
Melissa Stiebner,
Paula Maldonado,
Sandra Valdés,
Didier Marín,
Gerardo Delgado,
Virginia Laluz,
Lin-Bo Wu,
Paul Chavarriaga,
Joe Tohme,
Inez Slamet-Loedin,
Wolf Frommer
Affiliations
Van Luu
Institute for Molecular Physiology, Heinrich Heine University of Düsseldorf (HHU), Düsseldorf, GermanyMax-Planck-Institute for Plant Breeding Research (MPI), Köln, Germany
Melissa Stiebner
Institute for Molecular Physiology, Heinrich Heine University of Düsseldorf (HHU), Düsseldorf, Germany
Paula Maldonado
Institute for Molecular Physiology, Heinrich Heine University of Düsseldorf (HHU), Düsseldorf, Germany
Sandra Valdés
International Center for Tropical Agriculture (CIAT), PO Box 6713, Cali, Colombia
Didier Marín
International Center for Tropical Agriculture (CIAT), PO Box 6713, Cali, Colombia
Gerardo Delgado
International Center for Tropical Agriculture (CIAT), PO Box 6713, Cali, Colombia
Virginia Laluz
International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines
Lin-Bo Wu
Institute for Molecular Physiology, Heinrich Heine University of Düsseldorf (HHU), Düsseldorf, GermanyMax-Planck-Institute for Plant Breeding Research (MPI), Köln, Germany
Paul Chavarriaga
International Center for Tropical Agriculture (CIAT), PO Box 6713, Cali, Colombia
Joe Tohme
International Center for Tropical Agriculture (CIAT), PO Box 6713, Cali, Colombia
Inez Slamet-Loedin
International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines
Wolf Frommer
Institute for Molecular Physiology, Heinrich Heine University of Düsseldorf (HHU), Düsseldorf, GermanyMax-Planck-Institute for Plant Breeding Research (MPI), Köln, Germany, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Aichi 464-8602, Japan
Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both indica and japonica rice. Since breeders continuously develop new rice varieties, transformation protocols have to be adapted for each new variety. Here we provide an optimized transformation protocol with detailed tips and tricks for a new African variety Komboka using immature embryos. In Komboka, we obtained an apparent transformation rate of up to 48% for GUS/GFP reporter gene constructs using this optimized protocol. This protocol is also applicable for use with other elite indica rice varieties.
