Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells

Abstract

Objective To investigate the impact of exposure to 50 Hz magnetic field on transcription in Neuro2a/APP(695) cells. Methods Neuro2a/APP(695) cells were divided into radiation group and sham radiation group (n=3). The cells in the former group were exposed to 50 Hz magnetic field (1 mT, 4 h/d) for 3 consecutive days, and those from the latter group were only placed in the same coil system under the same conditions but without 50 Hz magnetic field. After exposure, all samples were collected to undergo standard procedures for transcriptome sequencing, including RNA extraction, data quality evaluation, and data analysis. Results There were 141 identified significant differentially expressed genes (DEGs) in Neuro2a/APP(695) cells after exposure, of which 129 were up-regulated and 12 were down-regulated. Gene Ontology (GO) enrichment analysis showed that the 62 up-regulated DEGs were involved in 432 significantly functional properties, of which 337 belong to biological processes, 16 to cellular components and 79 to molecular functions. While, the 3 down-regulated DEGs were involved in 275 significantly functional properties, of which 260 belonged to biological processes, 5 to cellular components and 10 to molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these DEGs may be involved in 20 differential KEGG pathways, including 2 down-regulated pathways and 18 up-regulated pathways. Conclusion Exposure to 50 Hz magnetic field may affect adhesion junction, Hippo signaling pathway, glycosaminoglycan biosynthesis-chondroitin sulfate/dermatan sulfate, protein digestion and absorption, mannose type O-glycan biosynthesis in Neuro2a/APP(695) cells, and these may be the main pathways that the exposure affects the cells.

Keywords

• 50 hz magnetic field
• neuro2a/app(695) cells
• mrna transcriptomics