METHODOLOGICAL AND CHEMICAL TEXTILE STUDY ON THE EFFECT OF METHONEIN AS ANTIBODY ANTIBODY IN THE TISSUE HERBAL TEXTILE

Abstract

This study conducted to induce the effect of DL-m~thionine as antioxidant on histochemistry as well as ability to induec histopathological changes in liver tissue of rabbit experimentally . In this study have used nine rabbit at weight rangine between 1100-1300 gm local breed ( 3 in each group) , divded into 3 groups 1st group consideredas control 2nd group treated with 0.4 % DL-methionine while 3rd group treated with 0.8% DL-methionine .Histological sections revealed histopathological lesions in liver at 0.8 % represented by vacuolar degeneration coagulative necrosis , portal fibrosis , hypertrophy in kupfer cells associated with hemosiderine pigmentation and invitation mononuclar inflammatony cells . In bile duct epithial hyperplasia , peri ductule cuffing of inflammatory cells have been seen.Histochemically , results showed sever positive reaction with PAS and BC , moderatia positive reaction with AB at pH 2.5 and BC. When estimation Glutathion (GSH) in liver tissue the results showed significant decrease in GSH concentration in liver tissue at 0.8% of methionine as compared with control group.

Keywords

• chemical textile
• methonein
• antibody