Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties

Abstract

Objective To prepare a new type of molybdenum disulfide (MoS2)/sodium alginate (SA) nanocomposite gel, and evaluate its microstructure, physical and chemical properties as well as its hemostatic and antibacterial performances. Methods The nanocomposite gel was prepared with a two-step method. Firstly, MoS2 nanosheets were synthesized by hydrothermal method; secondly, 2% SA solution was prepared as a template, being added with different concentrations of MoS2 dispersion; and then the SA/MoS2-X gel was obtained by crosslinking with calcium ions after being mixed evenly. The microstructure of SA and SA/MoS2-X were observed by scanning electron microscopy (SEM). Fourier transform infrared spectrometer (FTIR) analysis was used to analyze the surface functional groups of the substance through calibrating various vibration peaks on the spectrum. The absorption of the substance to specific wavelengths were measured by ultraviolet-visible (UV-Vis) light absorption spectroscopy. The inhibitory effects against E. coli and S. aureus and the hemostatic effect of SA/MoS2-X gel were evaluated by photothermal antibacterial experiment and rat liver hemorrhage model, respectively. Results The SEM results showed that the fiber network of the SA/MoS2-X gel was a 3-D porous structure, with its pore size and density being different from those of pure SA gel, and MoS2 was attached to the surface of SA gel and evenly distributed. The UV-Vis absorption spectrum indicated that the absorption peak of SA/MoS2-X was significantly higher than that of SA at 808 nm. The photothermal antibacterial effects against E.coli and S.aureus were remarkably enhanced in the SA/MoS2-X gel group (containing 1 and 2 mg/mL MoS2, respectively) (P < 0.05). In addition, the hemostatic effect of SA/MoS2-X gel on liver hemorrhage model of SD rats was greatly improved as compared with the control group (P < 0.05). Conclusion The SA/MoS2-X gel prepared by the two-step method has good porosity and binding stability to MoS2, with further improved photothermal and physicochemical properties, and presents high antibacterial performance against E.coli and S.aureus as well as good hemostatic effectiveness.

Keywords

• sodium alginate
• molybdenum disulfide
• photothermal antibacterial
• hemostatic gel