Efficacy of Ethanol Extract of Botanicals in Controlling Wheat Blast Fungus Magnaporthe oryzae triticum in vitro

Abstract

The present research work was conducted to evaluate the efficacy of eight fresh botanicals namely Azadirachta indica (Neem leaf), Allium cepa (Onion bulb), Allium sativum (Garlic), Curcuma longa (Turmeric), Zingiber officinale (Ginger), Allamanda cathartica (Allamanda leaf), Nigella sativa (Black cumin) and Aloe vera (Aloe vera) against Magnaporthe oryzae triticum (MoT) and solvent, ethanol (95%) was used for the phytochemical extraction of various plant parts. Three concentrations namely 1:1 (w/v), 1:0.50 (w/v) and 1:0.25 (w/v) of plant parts and ethanol were used for botanical extraction. The antifungal activity of botanicals against a virulent MoT isolate CHMoT07 was evaluated in-vitro using poison food technique. The lowest mycelia growth was recorded with Aloe vera (Aloe vera leaf) extracts and Nigella sativa (Black cumin seeds) extracts @ 1:1 w/v and @ 1:0.25 w/v with growth rate of 3.00 mm and 3.33 mm respectively at 7 days after inoculation, whereas the highest mycelia growth rate of MoT isolates was recorded in control plates both at 7 DAI and 14 DAI under in-vitro condition.

Keywords

• Wheat Blast
• Magnaporthe oryzae triticum
• botanical extracts
• in vitro