Optical Flow Cell for Measuring Size, Velocity and Composition of Flowing Droplets

Abstract

Here an optical flow cell with two light paths is reported that can accurately quantify the size and velocity of droplets flowing through a microchannel. The flow cell can measure the time taken for droplets to pass between and through two conjoined light paths, and thereby is capable of measuring the velocities (0.2–5.45 mm/s) and sizes of droplets (length > 0.8 mm). The composition of the droplet can also be accurately quantified via optical absorption measurements. The device has a small footprint and uses low-powered, low-cost components, which make it ideally suited for use in field-deployable and portable analytical devices.

Keywords

• droplet microfluidics
• absorbance
• real-time monitoring
• velocity measurement
• enzymatic assays
• optical detections