Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells

CHEN Xiaolin; ZHANG Menglin; CHANG Muyu; FANG Maocheng; HUANG Chuqing; QIU Xiaoyan; DENG Xinzhou; KE Qing; DUAN Qiwen; LUO Zhiguo

doi:10.3971/j.issn.1000-8578.2019.19.0305

Zhongliu Fangzhi Yanjiu (Oct 2019)

Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells

CHEN Xiaolin,
ZHANG Menglin,
CHANG Muyu,
FANG Maocheng,
HUANG Chuqing,
QIU Xiaoyan,
DENG Xinzhou,
KE Qing,
DUAN Qiwen,
LUO Zhiguo

Affiliations

CHEN Xiaolin: Department of Internal Medicine, First Clinical School, Hubei University of Medicine, Shiyan 442000, China
ZHANG Menglin: Department of Biological Sciences, School of Biological Engineering, Hubei University of Medicine, Shiyan 442000, China
CHANG Muyu: Department of Internal Medicine, First Clinical School, Hubei University of Medicine, Shiyan 442000, China
FANG Maocheng: Department of Internal Medicine, First Clinical School, Hubei University of Medicine, Shiyan 442000, China
HUANG Chuqing: Department of Internal Medicine, First Clinical School, Hubei University of Medicine, Shiyan 442000, China
QIU Xiaoyan: Department of Internal Medicine, First Clinical School, Hubei University of Medicine, Shiyan 442000, China
DENG Xinzhou: Cancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
KE Qing: Cancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
DUAN Qiwen: Cancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China
LUO Zhiguo: Cancer Prevention and Treatment Center, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China

DOI: https://doi.org/10.3971/j.issn.1000-8578.2019.19.0305
Journal volume & issue: Vol. 46, no. 10
pp. 873 – 878

Abstract

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Objective To investigate the effects of curcumin on epithelial mesenchymal transition of non-small cell lung cancer (NSCLC) A549 cell line induced by ionizing radiation. Methods The epithelial mesenchymal transformation of A549 cells was induced by ionizing radiation and named as A549R cells. The proliferation of A549 cells was detected by CCK8 assay. The expression levels of epithelial and mesenchymal markers were detected by Western blot in A549R cells treated with curcumin. The effects of curcumin on the migration ability of A549R cells was detected by scratch test and Transwell assay. Results After curcumin treatment, the morphology of A549R cells changed from spindle to oval epithelium; the expression level of E-cadherin was down-regulated, while the expression of pan-Keratin was up-regulated; the expression of Vimentin and Twist were significantly down-regulated, while the expression level of N-cadherin was not significantly changed. Scratch healing area of scratches and cell migration ability were decreased significantly with the increased concentration of curcumin in A549R cells. Conclusion Curcumin reverses the epithelial mesenchymal transformation of A549 cells induced by ionizing radiation by down-regulating the expression of E-cadherin, vimentin, Twist and up-regulating the expression of Keratin.

Published in Zhongliu Fangzhi Yanjiu

ISSN: 1000-8578 (Print)
Publisher: Magazine House of Cancer Research on Prevention and Treatment
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.zlfzyj.com

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