Dietary supplementation of papaya (Carica papaya l.) leaf affects abundance of Butyrivibrio fibrisolvens and modulates biohydrogenation of C18 polyunsaturated fatty acids in the rumen of goats

Abstract

Saturated fatty acid (SFA) produced as a final product of rumen biohydrogenation (BH) by rumen microbes is harmful to human health because of increasing the risk of heart-related diseases. The objectives of this study were to test the effect of papaya leaf (PL) on the BH of C18 polyunsaturated fatty acids (PUFA), fatty acid composition and bacterial abundance in the rumen of goats. Three rumen fistulated male goats were assigned in a 3 × 3 latin square design using three levels of PL; no addition of PL in basal diet (control, CON, 50% concentrate +50% alfalfa hay), 25% of alfalfa hay in basal diet replaced by PL (medium PL, MPL) and 50% of alfalfa hay in basal diet replaced by PL (high PL, HPL). Supplementation of the diet with PL significantly (p < .05) decreased the rate of rumen BH of C18:2n-6 (linoleic acid, LA) at different hours of sampling at the higher inclusion of PL (HPL), resulted in the reduction (p < .05) of C18:0 (stearic acid, SA) in the rumen liquor. The population of Butyrivibrio fibrisolvens as determined by the real-time PCR was higher (p < .05) for MPL (5.78 cell/mL) and HPL (6.09 cell/mL) in the rumen of goats as compared with the CON (5.50 cell/mL). The current results imply that PL supplementation could be a useful feeding strategy to modulate the BH of LA in rumen of goat.

Keywords

• biohydrogenation
• c18 pufa
• fatty acid
• goat
• papaya leaf
• rumen