Evaluation of NQO1 Polymorphism in Gastric Adenocarcinoma

Abstract

Background and purpose: Gastric cancer is the second most common cause of cancer worldwide and despite huge advances in treatments, the chance of survival is very low even in surgery cases. Having a genetic predisposition plays an important role in cancer development. Polymorphisms in the NQO1(NAD(P)H Quinone dehydrogenase 1) gene could affect the activity of the gene and also the susceptibility to gastric cancer. The aim of this study was to evaluate the effect of the polymorphism of NQO1 gene on gastric cancer. Materials and methods: This case-control study was performed in 100 patients with gastric cancer and 100 healthy individuals as benchmarks. Genomic DNA was extracted from peripheral blood leukocytes. Then, genotyping for detecting NQO1 -609 C>T promoter gene polymorphism was carried out using PCR-RFLP. Data analysis was performed in MedCalc V9. Results: NQO1 rs1800566 allelic and genotypic frequencies were not significantly different between the patients and controls (P= 0.14، χ2= 2.16( Conclusion: Screening of -609C>T NQO1 polymorphism was not found as a useful marker in detecting personal sensitivity to gastric cancer, therefore, would not be helpful in gastric cancer prevention and treatment processes.

Keywords

• nqo1 promoter
• gastric cancer
• genetic polymorphism
• pcr
• rflp-pcr