Background/Objectives: Using advanced methodologies may enhance athlete profiling. This study profiled morphological and laboratory-derived performance biomechanics by position of American-style football players training for the draft. Methods: Fifty-five players were categorized into three groups: Big (e.g., lineman; n = 17), Big–skill (e.g., tight end; n = 11), and Skill (e.g., receiver; n = 27). Body fat (BF%), lean body mass (LBM), and total body mass were measured using a bioelectrical impedance device. Running ground reaction force (GRF) and ground contact time (GCT) were obtained using an instrumented treadmill synchronized with a motion capture system. Dual uniaxial force plates captured countermovement jump height (CMJ-JH), normalized peak power (CMJ-NPP), and reactive strength. Asymmetry was calculated for running force, GCT, and CMJ eccentric and concentric impulse (IMP). MANOVA determined between-group differences, and radar plots for morphological and performance characteristics were created using Z-scores. Results: There was a between-group difference (F(26,80) = 5.70, p 2 = 0.649). Fisher’s least squares difference post hoc analyses showed that participants in the Skill group had greater JH, CMJ-NPP, reactive strength, and running GRF values versus Big players but not Big–skill players (p p p p > 0.05). Morphological and performance biomechanics differences are pronounced between Skill and Big players. Big–skill players possess characteristics from both groups. Laboratory-derived metrics offer precise values of running and jumping force strategies and body composition that can aid sports science researchers and practitioners in refining draft trainee profiles.
