CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Miguel A. Moreno-Mateos; Juan P. Fernandez; Romain Rouet; Charles E. Vejnar; Maura A. Lane; Emily Mis; Mustafa K. Khokha; Jennifer A. Doudna; Antonio J. Giraldez

doi:10.1038/s41467-017-01836-2

Nature Communications (Dec 2017)

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Miguel A. Moreno-Mateos,
Juan P. Fernandez,
Romain Rouet,
Charles E. Vejnar,
Maura A. Lane,
Emily Mis,
Mustafa K. Khokha,
Jennifer A. Doudna,
Antonio J. Giraldez

Affiliations

Miguel A. Moreno-Mateos: Department of Genetics, Yale University School of Medicine
Juan P. Fernandez: Department of Genetics, Yale University School of Medicine
Romain Rouet: Department of Molecular and Cell Biology, University of California
Charles E. Vejnar: Department of Genetics, Yale University School of Medicine
Maura A. Lane: Department of Genetics, Yale University School of Medicine
Emily Mis: Department of Genetics, Yale University School of Medicine
Mustafa K. Khokha: Department of Genetics, Yale University School of Medicine
Jennifer A. Doudna: Department of Molecular and Cell Biology, University of California
Antonio J. Giraldez: Department of Genetics, Yale University School of Medicine

DOI: https://doi.org/10.1038/s41467-017-01836-2
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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Cpf1 is a promising addition to the CRISPR toolkit but displays wide variability of activity in different eurkaryotes. Here the authors identify temperature as a modulator of activity and use this to efficiently edit ectothermic vertebrate species.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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