Rapid and specific detection of Trichophyton rubrum and Trichophyton mentagrophytes using a loop-mediated isothermal amplification assay
Mi-Ran Seo,
Hyo Seok Kim,
Young Bok Lee,
Sun Shin,
Yeun-Jun Chung
Affiliations
Mi-Ran Seo
ConnectaGen, Hanam 12918, Korea
Hyo Seok Kim
ConnectaGen, Hanam 12918, Korea
Young Bok Lee
Department of Dermatology, Uijeongbu St. Mary's Hospital, The Catholic University of Korea, Uijeongbu, Korea; Department of Biomedicine & Health Sciences
Sun Shin
Department of Microbiology; Precision Medicine Research Center
Yeun-Jun Chung
Department of Biomedicine & Health Sciences; Department of Microbiology; Precision Medicine Research Center; IRCGP, The Catholic University of Korea, Seoul 06591, Republic of Korea; Corresponding author: Yeun-Jun Chung M.D., Ph.D., Precision Medicine Research Center, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea
Trichophyton rubrum and Trichophyton mentagrophytes are the main causative pathogens of onychomycosis. However, the sensitivity and specificity of conventional microscopic tests are insufficient for reliable diagnoses of onychomycosis. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for the rapid and specific identification of the two major Trichophytons spp. We designed LAMP primers targeting the internal transcribed spacer 1 region of the T. rubrum and T. mentagrophytes. Through rigorous optimization of the reaction conditions, we defined a universal reaction condition for both LAMP assays.
