The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro

Abstract

The influence of a low-molecular fraction (below 5 kDa) from the cattle cord blood (CBF) on functional activity of phagocytes, human embryonic fibroblasts, mesenchymal stromal cells and BHK-21 clone 13/04 and PK-15 cells was studied. The low-molecular fraction added to culture medium increases the growth rate of cell cultures. The incubation of leukoconcentrate in the CBF-containing medium results in an increase in phagocytic indices of neutrophils in the presence of a phagocytosis inhibitor – sodium iodoacetate, leading to a significant increase in intracellular glucose content and alkaline phosphatase activity as compared to the control and the reference drug Actovegin®.

Keywords

• Actovegin®
• alkaline phosphatase
• BHK-21 clone 13/04
• cord blood low-molecular fraction
• glucose
• human embryonic fibroblasts
• inhibitors of phagocytosis
• leukocytes
• mesenchymal stromal cells
• PK-15 cells