LEVEL OF LOCAL PRODUCTION OF CYTOKINES IN THE CLINIC OF TRADITIONAL TREATMENT OF DEMODECTIC BLEPHARITIS AND IN CONDITIONS OF LID CRYOTHERAPY USE

Abstract

Aim. A comparative cytokine profile assessment of the lacrimal fluid of the eyes of patients in the clinic of traditional treatment of demodectic blepharitis, and using the course of cryotherapy of the eyelids by an autonomous cryoapplicator made of titanium nickelide. Materials and methods. Clinical studies were conducted in 65 patients with demodectic blepharoconjunctivitis (DBC). A tear of the eye was taken on the 3rd, 7th and 30th days after the traditional treatment, and using cryotherapy of the eyelids by an autonomous cryoapplicator made of porous permeable titanium nickelide cooled in liquid nitrogen. In the studied biological fluid (tear) of patients with DBC, the content of cytokines (IL1β, IL8, IFNα, IL2, IL10) was evaluated by an enzyme immunoassay. Results. The opposite nature of the changes in the balance of cytokines and the ratio of its pro and anti-inflammatory links in the time of observation was revealed. A significant dynamic increase of IFNa and IL2 content in the lacrimal fluid of the examined after cryostimulation can evidence for an adequate activation of cellular immune link under its interaction with Demodex mite antigens, as well as for reinforcing the regeneration mechanisms. Conclusion. The analysis of obtained data evidences for the high efficiency of clinical and immunological effect of lid cryotherapy method for DBC treatment.

Keywords

• cytokines
• patients’ tear
• eye demodecosis
• cryoprobe of titanium nickelide