Micro propagation technique in Kinnow mandarin (Citrus reticulata)

Abstract

Study on standardization of micro propagation technique in Kinnow mandarin was carried out using nodal segments of nucelles seedling as explants. The MS medium supplemented with BAP, NAA and IAA for shoot regeneration and IBA and NAA for rooting were used in different concentration either alone or in combinations. The minimum time required to bud break (15.80 days) required was noticed on MS medium modified supplemented with BAP 1.0 mg/l + IAA 0.5 mg/l. The maximum number of shoot (4.90) on 1.0 mg/l BAP + 0.5 mg/l NAA and length of shoot (2.62 cm) on BAP 1.0 mg/l + IAA 0.5 mg/l. The maximum per cent (90 %) of micro shoots responded to rooting was observed in medium containing 1.0 mg/l either IBA or NAA or in combination of NAA (0.5 mg/l) + IBA (0.1 mg/l). The minimum time required to root induction (18.40 days) was recorded with NAA (0.5 mg/l) + IBA (0.5 mg/l). The maximum number of roots (6.70) and length (4.40 cm) were observed on MS medium supplemented with NAA (0.5 mg/l) + IBA (0.5 mg/l). The rooted plantlets were successfully acclimatized in a green house, in pots containing sterile soil, perlite and vermiculite in equal proportion. After 60 days of acclimatization under greenhouse conditions, 85.0 per cent success was noted.

Keywords

• Acclimatization
• In vitro
• Kinnow
• Micro propagation
• Nucellar