Isolation of S-Layer from Caulobacter and re-crystallization on planar supports

Abstract

 Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-crystallization after isolation from the surface of bacteria and therefore could be used for fabrication of assembled functional groups. Materials and methods: To this regard in this work S- Layers were isolated from Caulobacter and were assembled on mica and silicon wafers. S-Layers re-crystallization were characterized by means of AFM. In order to evaluate the production of assembled functional groups Immunological methods were used. Results: S-Layer with molecular weight of 96 KDa were successfully isolated from the surface of Caulobacter and crystallized on solid silicone and mica support. AFM results indicated assembled domains on mica and silicone. IgG immobilization was induced in the presence of S-Layer structures. Discussion and conclusion: The results confirmed the effect of S-Layer crystallization on fabrication of assembled functional groups.

Keywords

• Self- assembly
• S- Layer
• Functional group