OncoTargets and Therapy (Jul 2018)

Polymorphism of IFN-γ+874T/A associated with production of IFN-γ affects human papillomavirus susceptibility in rural women from Luohe, Henan, China

  • Zhang QW,
  • Song JY,
  • Yu JH,
  • Sun MZ,
  • Tang SY,
  • Yang SZ,
  • Cao LJ,
  • Wang HF,
  • Cui LN,
  • Fu XH

Journal volume & issue
Vol. Volume 11
pp. 4339 – 4344

Abstract

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Qing-wei Zhang,1 Jia-yu Song,2 Jiang-hua Yu,1 Ming-zhen Sun,2 Si-yuan Tang,1 Shao-zhe Yang,1 Lei-jia Cao,1 Hui-fen Wang,1 Li-na Cui,1 Xiu-hong Fu1 1Department of Obstetrics and Gynaecology, Luohe Central Hospital, Luohe, 462000, Henan, China; 2Department of Pharmacology, Luohe Medical College, Luohe, 462000, Henan, China Purpose: In this paper, the association between polymorphisms of IFN-γ+874T/A (rs2430561), IFN-γR1 -56 T/C (rs2234711), IFN-γR1 +95 C/T (rs7749390), and IFN-γR1 -611A/G (rs 1327474) and human papillomavirus (HPV) susceptibility was investigated in rural women from Luohe, Henan, China. Patients and methods: A total of 520 rural women were enrolled from Luohe, including 260 with HPV infection and mild dysplasia or less and 260 without HPV infection. Single-nucleotide polymorphisms (SNPs) of IFN-γ +874T/A, IFN-γR1 -56 T/C, IFN-γR1 +95 C/T and IFN-γR1 -611A/G were genotyped using TaqMan Pre-Designed SNP Genotyping Assays. Serum IFN-γ levels were measured using Human IFN-γ Quantikine ELISA Kit. Multivariate logistic regression analysis was performed to identify the SNPs associated with HPV susceptibility. Serum IFN-γ levels were compared between different genotypes. Results: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility and +874A carriers had an increased risk. Moreover, the odds ratio was higher in +874 AA carriers than in +874 AT carriers (1.672 vs 2.874). Serum IFN-γ levels were highest in IFN-γ +874 TT carriers, intermediate in AT carriers, and lowest in AA carriers (2.86±1.14 vs 1.57±0.79 vs 0.41±0.22 pg/mL, all P<0.05). Conclusion: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility in rural women from Luohe, Henan, China, and +874A carriers had an increased risk. The possible mechanism was that +874A carriers had a low production of IFN-γ. Keywords: single-nucleotide polymorphisms, IFN-γ, IFN-γR1, human papillomavirus, susceptibility

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