BMC Research Notes (Oct 2009)

Observational study on variability between biobanks in the estimation of DNA concentration

  • McArdle Wendy,
  • Lewis David H,
  • Laiho Päivi,
  • Lagerberg Camilla,
  • Krinka Dagni,
  • Ke Xiayi,
  • Illig Thomas,
  • Hardy Matthew,
  • Gwilliam Rhian,
  • Foedinger Manuela,
  • Dixon Katherine P,
  • Bracegirdle Pippa,
  • Aslanidis Charalampos,
  • Donev Alexander N,
  • Brown Jay,
  • Patton Simon,
  • Ring Susan M,
  • Schmitz Gerd,
  • Stevens Helen,
  • Tybring Gunnel,
  • Wichmann H Erich,
  • Ollier William ER,
  • Yuille Martin A

DOI
https://doi.org/10.1186/1756-0500-2-208
Journal volume & issue
Vol. 2, no. 1
p. 208

Abstract

Read online

Abstract Background There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. Methods DNA aliquots at nominal concentrations between 10 and 300 ng/μl were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Results Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Conclusion Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation.