Improved Affinity: A Customized Fluorescent Probe for the Rapid Detection of Butyrylcholinesterase
Wei Wang,
Xiao-Fei Chen,
Yi Zhang,
Yang Ran,
Long Jin,
Shuai Li,
Bai-Ou Guan
Affiliations
Wei Wang
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Xiao-Fei Chen
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Yi Zhang
College of Life Science and Technology, Jinan University, Guangzhou 510632, China
Yang Ran
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Long Jin
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
Shuai Li
Special Needs Medical Center, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, China
Bai-Ou Guan
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China
This article presents the distinctive butyrylcholinesterase (BChE) fluorescent probe P5, designed via a targeting-site method. This method was proposed to enhance the affinity of the probe for BChE by targeting the peripheral anionic site (PAS) of BChE. By mimicking the natural substrate butyrylcholine, the structure of the probe was optimized by introducing a positive charge. Fluorescent probe P5, selected from a series of designed fluorescent probes P1–P6, exhibited excellent affinity and specificity towards BChE, enabling rapid detection within 5 min with a low detection limit of 16.7 ng/mL. Furthermore, this probe can distinguish between normal cells and Alzheimer’s disease (AD) model cells, and demonstrated good imaging results in a P12 cell AD model. The results of this study indicate that this novel fluorescent probe could serve as a promising tool for the rapid detection of BChE and accurate AD diagnosis.
