Culture-independent genome sequencing of Coxiella burnetii from a native heart valve of a Tunisian patient with severe infective endocarditis

J. Delaloye; T. Pillonel; M. Smaoui; A. Znazen; L. Abid; G. Greub

New Microbes and New Infections (Jan 2018)

Culture-independent genome sequencing of Coxiella burnetii from a native heart valve of a Tunisian patient with severe infective endocarditis

J. Delaloye,
T. Pillonel,
M. Smaoui,
A. Znazen,
L. Abid,
G. Greub

Affiliations

J. Delaloye: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland; Intensive Care Unit, Department of Intensive Care Medicine, University of Lausanne and University Hospital Center, Lausanne, Switzerland
T. Pillonel: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
M. Smaoui: Laboratory of Microbiology, CHU Habib Bourguiba Sfax, University of Sfax, Tunisia
A. Znazen: Laboratory of Microbiology, CHU Habib Bourguiba Sfax, University of Sfax, Tunisia
L. Abid: Department of Cardiology, Hedi Chaker University Hospital, University of Sfax, Sfax, Tunisia
G. Greub: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland; Corresponding author: G. Greub, Institute of Microbiology, University of Lausanne and University Hospital Center, Rue du Bugnon 48, 1011 Lausanne, Switzerland

Journal volume & issue: Vol. 21
pp. 31 – 35

Abstract

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We report draft genome of a Coxiella burnetii strain sequenced from the native valve of a patient presenting with severe endocarditis in Tunisia. The genome could be sequenced without a cellular or axenic culture step. The MST5 strain was demonstrated to be closely related to the published reference genome of C. burnetii CbuK_Q154. Keywords: Cardiac valve, endocarditis, genomics, intracellular bacteria

Published in New Microbes and New Infections

ISSN: 2052-2975 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.sciencedirect.com/journal/new-microbes-and-new-infections

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