Photodynamic Therapy Using a Rose-Bengal Photosensitizer for Hepatocellular Carcinoma Treatment: Proposition for a Novel Green LED-Based Device for In Vitro Investigation
Anthony Lefebvre,
Smail Marhfor,
Gregory Baert,
Pascal Deleporte,
Guillaume Paul Grolez,
Marie Boileau,
Olivier Morales,
Séverine Vignoud,
Nadira Delhem,
Laurent Mortier,
Anne-Sophie Dewalle
Affiliations
Anthony Lefebvre
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Smail Marhfor
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Gregory Baert
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Pascal Deleporte
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Guillaume Paul Grolez
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Marie Boileau
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Olivier Morales
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Séverine Vignoud
CEA, LETI, University of Grenoble Alpes, 38000 Grenoble, France
Nadira Delhem
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Laurent Mortier
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Anne-Sophie Dewalle
Inserm, CHU Lille, U1189—ONCO-THAI—Assisted Laser Therapy and Immunotherapy for Oncology, University of Lille, 59000 Lille, France
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Despite new treatments, the HCC rate remains important, making it necessary to develop novel therapeutic strategies. Photodynamic therapy (PDT) using a Rose-Bengal (RB) photosensitizer (RB-PDT) could be a promising approach for liver tumor treatment. However, the lack of standardization in preclinical research and the diversity of illumination parameters used make comparison difficult across studies. This work presents and characterizes a novel illumination device based on one green light-emitting diode (CELL-LED-550/3) dedicated to an in vitro RB-PDT. The device was demonstrated to deliver a low average irradiance of 0.62 mW/cm2 over the 96 wells of a multi-well plate. Thermal characterization showed that illumination does not cause cell heating and can be performed inside an incubator, allowing a more rigorous assessment of cell viability after PDT. An in vitro cytotoxic study of the RB-PDT on an HCC cell line (HepG2) demonstrated that RB-PDT induces a significant decrease in cell viability: almost all the cells died after a light dose irradiation of 0.3 J/cm2 using 75 µM of RB (<10% of viability). In conclusion, the RB-PDT could be a therapeutic option to treat unresectable liver lesions and subclinical disease remaining in the post-resection tumor surgical margin.
