Zhongguo youzhi (Jan 2023)
基于广泛靶向代谢组学分析黑老虎种子中 氨基酸和脂质组分Analysis of amino acids and lipids in Kadsura coccinea seed by widely targeted metabolomics
Abstract
为探究黑老虎种子作为新兴植物油料资源的潜力，利用广泛靶向代谢组学技术检测分析其氨基酸和脂质组分。结果表明：在基于鉴定出的代谢物结构配置分类下，黑老虎种子含脂质类、氨基酸及衍生物类化合物分别为121种和89种，前者数量和相对含量（30.7%）在11类成分中最高，后者数量居其次，相对含量（17.8%）位于第三；鉴定出20种基础氨基酸和8种人体必需氨基酸，其中L-脯氨酸、L-天冬氨酸、L-丝氨酸等7种药效氨基酸和L-亮氨酸、L-苏氨酸、L-异亮氨酸、L-苯丙氨酸和L-赖氨酸5种人体必需氨基酸含量较高；黑老虎种子中富含亚油酸、γ-亚麻酸、α-亚麻酸等脂肪酸和以溶血磷脂酰胆碱18∶ 2和18∶ 2（2n异构）为主的磷脂。黑老虎种子氨基酸类和脂质类组分丰富，多种高度富集成分具有突出的营养功能、活性作用和药用功效，在优质植物油脂开发等领域具有较高的价值。 In order to explore the potential of Kadsura coccinea seed as a new plant oil resource, the amino acids and lipids were detected and analyzed by widely targeted metabolomics. The results showed that under the classification based on the identified metabolite structure configuration, the Kadsura coccinea seed contained 121 lipid and 89 amino acid and derivative compounds, respectively, and the number and the relative content (30.7%) of the former was the highest among the 11 types of components, the number of the latter was the second, and the relative content (17.8%) was the third. 20 kinds of basic amino acids and 8 kinds of essential amino acids were identified. The contents of 7 kinds of medicinal amino acids (such as L-proline, L-aspartic acid and L-serine) and 5 kinds of essential amino acids (such as L-leucine, L-threonine, L-isoleucine, L-phenylalanine and L-lysine) were higher. Kadsura coccinea seed was rich in fatty acids such as linoleic acid, γ-linolenic acid and α-linolenic acid, as well as phospholipids dominated by lysophosphatidylcholines 18∶ 2 and 18∶ 2 (2n isomer). In conclusion，Kadsura coccinea seed is rich in amino acids and lipids, and a variety of highly enriched components have outstanding nutritional functions, activities and medicinal effects, and have high value in the development of high-quality vegetable oils and other fields.
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