Structural Elucidation of a Polysaccharide from <i>Flammulina velutipes</i> and Its Lipid-Lowering and Immunomodulation Activities

Wei Jia; Wenhan Wang; Dongsheng Yu; Yangchao Yu; Zhan Feng; Hewen Li; Jingsong Zhang; Henan Zhang

doi:10.3390/polym16050598

Polymers (Feb 2024)

Structural Elucidation of a Polysaccharide from <i>Flammulina velutipes</i> and Its Lipid-Lowering and Immunomodulation Activities

Wei Jia,
Wenhan Wang,
Dongsheng Yu,
Yangchao Yu,
Zhan Feng,
Hewen Li,
Jingsong Zhang,
Henan Zhang

Affiliations

Wei Jia: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, P. R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China
Wenhan Wang: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, P. R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China
Dongsheng Yu: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, P. R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China
Yangchao Yu: Jiangsu Chinagreen Biotechnology Co., Ltd., Suqian 223700, China
Zhan Feng: Jiangsu Chinagreen Biotechnology Co., Ltd., Suqian 223700, China
Hewen Li: Jiangsu Chinagreen Biotechnology Co., Ltd., Suqian 223700, China
Jingsong Zhang: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, P. R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China
Henan Zhang: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, P. R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China

DOI: https://doi.org/10.3390/polym16050598
Journal volume & issue: Vol. 16, no. 5
p. 598

Abstract

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FVPT1, a novel heteropolysaccharide, was purified from the fruiting body of Flammulina velutipes using magnetic-field-assisted three-phase partitioning and gel permeation chromatography. The structure was characterized using monosaccharide composition and methylation analysis, infrared spectroscopy and nuclear magnetic resonance (NMR). The FVPT1 (~1.64 × 104 Da) was composed of L-fucose, D-galactose, D-glucose and D-mannose at a molar ratio of 1.0:3.5:1.0:1.4. The polysaccharide repeating unit of FVPT1 was established with methylation analyses and NMR spectroscopy. Moreover, a zebrafish larva hyperlipidemia model test demonstrated that FVPT1 can show appreciable lipid-lowering effects. In addition, the FVPT1 exhibited remarkable immunoregulatory activity by increasing nitric oxide, interleukin (IL)-1β and IL-1 secretion in macrophages. Therefore, these results suggest that FVPT1 has the potential to be developed into a new immune or hypolipidemic health product.

Published in Polymers

ISSN: 2073-4360 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/polymers/

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