STAR Protocols (Jun 2021)

Cell population analysis of the adult murine subependymal neurogenic lineage by flow cytometry

  • Germán Belenguer,
  • Pere Duart-Abadia,
  • Ana Domingo-Muelas,
  • Jose Manuel Morante-Redolat,
  • Isabel Fariñas

Journal volume & issue
Vol. 2, no. 2
p. 100425

Abstract

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Summary: This protocol provides a flow-cytometry-based procedure to classify and isolate all cells of the adult rodent subependymal zone (SEZ) neurogenic lineage, without the need for reporter mice, into different cell populations, including three neural stem cell (NSC) fractions with molecular signatures that are coherent with single-cell transcriptomics. Additionally, their cycling behavior can be assessed by means of 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Our method allows the isolation of different NSC fractions and the functional assay of their cycling heterogeneity and quiescence-activation transitions.For complete details on the use, execution, and outcomes of this protocol, please refer to Belenguer et al. (2021).

Keywords