Nature Communications (Jun 2016)
Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification
- Wim Dejonghe,
- Sabine Kuenen,
- Evelien Mylle,
- Mina Vasileva,
- Olivier Keech,
- Corrado Viotti,
- Jef Swerts,
- Matyáš Fendrych,
- Fausto Andres Ortiz-Morea,
- Kiril Mishev,
- Simon Delang,
- Stefan Scholl,
- Xavier Zarza,
- Mareike Heilmann,
- Jiorgos Kourelis,
- Jaroslaw Kasprowicz,
- Le Son Long Nguyen,
- Andrzej Drozdzecki,
- Isabelle Van Houtte,
- Anna-Mária Szatmári,
- Mateusz Majda,
- Gary Baisa,
- Sebastian York Bednarek,
- Stéphanie Robert,
- Dominique Audenaert,
- Christa Testerink,
- Teun Munnik,
- Daniël Van Damme,
- Ingo Heilmann,
- Karin Schumacher,
- Johan Winne,
- Jiří Friml,
- Patrik Verstreken,
- Eugenia Russinova
Affiliations
- Wim Dejonghe
- Department of Plant Systems Biology
- Sabine Kuenen
- VIB Center for the Biology of Disease, Laboratory of Neuronal Communication
- Evelien Mylle
- Department of Plant Systems Biology
- Mina Vasileva
- Institute of Science and Technology Austria
- Olivier Keech
- Department of Plant Physiology, Umeå Plant Science Centre, Umeå University
- Corrado Viotti
- Department of Plant Physiology, Institute of Biochemistry and Biology, University of Potsdam
- Jef Swerts
- VIB Center for the Biology of Disease, Laboratory of Neuronal Communication
- Matyáš Fendrych
- Institute of Science and Technology Austria
- Fausto Andres Ortiz-Morea
- Department of Plant Systems Biology
- Kiril Mishev
- Department of Plant Systems Biology
- Simon Delang
- Developmental Biology of Plants, Centre for Organismal Studies, Heidelberg University
- Stefan Scholl
- Developmental Biology of Plants, Centre for Organismal Studies, Heidelberg University
- Xavier Zarza
- Department of Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam
- Mareike Heilmann
- Department of Cellular Biochemistry, Institute for Biochemistry and Biotechnology, Martin-Luther-University
- Jiorgos Kourelis
- Department of Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam
- Jaroslaw Kasprowicz
- VIB Center for the Biology of Disease, Laboratory of Neuronal Communication
- Le Son Long Nguyen
- Compound Screening Facility
- Andrzej Drozdzecki
- Compound Screening Facility
- Isabelle Van Houtte
- Department of Plant Systems Biology
- Anna-Mária Szatmári
- Department of Plant Systems Biology
- Mateusz Majda
- Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences
- Gary Baisa
- Department of Biochemistry, University of Wisconsin-Madison
- Sebastian York Bednarek
- Department of Biochemistry, University of Wisconsin-Madison
- Stéphanie Robert
- Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences
- Dominique Audenaert
- Compound Screening Facility
- Christa Testerink
- Department of Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam
- Teun Munnik
- Department of Plant Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam
- Daniël Van Damme
- Department of Plant Systems Biology
- Ingo Heilmann
- Department of Cellular Biochemistry, Institute for Biochemistry and Biotechnology, Martin-Luther-University
- Karin Schumacher
- Developmental Biology of Plants, Centre for Organismal Studies, Heidelberg University
- Johan Winne
- Department of Organic and Macromolecular Chemistry, Laboratory for Organic Synthesis, Ghent University
- Jiří Friml
- Institute of Science and Technology Austria
- Patrik Verstreken
- VIB Center for the Biology of Disease, Laboratory of Neuronal Communication
- Eugenia Russinova
- Department of Plant Systems Biology
- DOI
- https://doi.org/10.1038/ncomms11710
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 12
Abstract
Plant cells maintain strict proton gradients over different membranes. Here, Dejonghe et al. show that several protonophores, including the known tyrosine kinase inhibitor TyrphostinA23, inhibit clathrin-mediated endocytosis by disturbing these gradients and causing cytoplasmic acidification.
