The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis

Abstract

The aim of this study was to evaluate the level of secretion of IL-12β by mononuclear leukocytes of peripheral blood under infiltrative and disseminated pulmonary tuberculosis. Blood mononuclear cells were isolated by gradient centrifugation, then cultured in complete culture medium; the level of IL-12β production was determined by the ELISA (ELISA). Peripheral blood served as the material of the research. It has been established, that under infiltrative pulmonary tuberculosis the levels of basal, protein and lipid-induced IL-12β production were significantly lower than in healthy donors and patients with disseminated pulmonary tuberculosis. Under disseminated pulmonary tuberculosis basal IL-12β production varies within normal limits; when stimulated by protein antigen, it increases, when influenced by lipid antigen — decreases.

Keywords

• pulmonary tuberculosis
• antigen-presenting cells
• il-12β