Kesesuaian antara Metode Microscopic Observation Drug Susceptibility Assay dan Ogawa pada Biakan Mycobacterium tuberculosis

Abstract

Tuberculosis (TB) is a problem of public health that causing high morbidity and mortality rates in various countries in the world. The diagnosis of pulmonary tuberculosis in adults can be established based on the discovery of M. tuberculosis on smear or culture of sputum. Culture is the gold standard but the availlable method is time consuming, it is need minimal eight weeks. Microscopic observation drug susceptibility assay (MODS) is one of methods for M. tuberculosis culture using liquid medium that can be a simultaneously test for M. tuberculosis drug sensitivity. The purpose of this study was to determine the conformity of the MODS method compared with Ogawa method for cultivation of M. tuberculosis in pulmonary TB patients. The cross sectional research has been conducted at Clinical Pathology Department of Dr. Hasan Sadikin Hospital, Bandung and examination of the specimen done at Health Laboratory Development Unit (BPLK), West Java Province between April to August 2010. The subjects were patients who diagnosed as pulmonary TB suspect. Each collected specimen was cultured in liquid media MODS and solid media Ogawa. To analyze the conformity of MODS and Ogawa method, Kappa coefficient of agreement was used. There were 172 specimens collected from 133 subjects. The conformity between culture results of M. tuberculosis in MODS method and in Ogawa’s method using Kappa coefficient, was high (Kappa index 0.91). The difference of growth time of M. tuberculosis significant (p=0.000), in MODS was 10.1 days (range 4–21 days) and in Ogawa method was 24.8 days (range 14–35 days). Conclusion, MODS and Ogawa’s method have the relatively similar diagnostic success rate, the advantage of MODS method is, the growth of M. tuberculosis is faster than in Ogawa method.

Keywords

• Microscopic observation drug susceptibility assay (MODS)
• M. tuberculosis
• Ogawa
• sputum