Biogenic Silver Nanoparticles (AgNPs) from Marphysa moribidii Extract: Optimization of Synthesis Parameters

Abstract

Interest in biogenic silver nanoparticles (AgNPs) is steadily increasing due to the cost-effective, easy, and environmentally friendly way in which they are synthesized. Synthesis using polychaete (Marphysa moribidii) extract as a reducing agent is particularly new and has the potential of being applied in various industries. However, biogenic AgNPs require synthesis optimization to increase their stability, yield, and characteristics. To meet these requirements, several synthesis parameters (such as polychaete size (body width), silver nitrate (AgNO3) concentration, pH of polychaete crude extract, and the temperature during pre-incubation) and storage conditions were optimized in this study. The optimized conditions for obtaining high yield and stable AgNPs were polychaetes with a body width of 6–8 mm, 1 mM AgNO3 with polychaete crude extract of pH 9, preheated at 90°C for 15 min before incubation at 30°C (150 rpm) for 24 hours, and stored at 4°C for long-term stability. The formation of AgNPs was confirmed through observation of a color transition (from pinkish to yellowish-brown) and analysis of UV-Vis spectra (between 398 and 400 nm). Scanning electron microscopy and transmission electron microscopy revealed the formation of spherical AgNPs with an average size of approximately 40.19 nm. Further, the optimized AgNPs demonstrated high storage stability for up to 6 months without any agglomeration. It is believed that these parameters are eminently suitable for the production of stable biosynthesized AgNPs.

Keywords

• biosynthesis
• marphysa moribidii
• optimization
• polychaetes
• silver nanoparticles