Application of Crustaceans as Ecological Markers for the Assessment of Pollution of Brackish Lakes of Bulgaria Based on Their Ability to Accumulate the Heavy Metals Cd, Zn and Ni

Abstract

The present study aimed to assess the pollution of Bulgarian brackish lakes based on their ability to accumulate the heavy metals Cd, Zn and Ni. Physicochemical parameters, including pH, electrical conductivity and salinity of the waters, were determined by potentiometric methods. The heavy metal content of the water and crustacean samples was determined by atomic absorption spectrophotometry. The highest pH in the investigated lakes (Atanasovsko Lake, Poda and Pomorie Lake) in the period May–September 2021 was found in the month of September, in the waters of Atanasovsko Lake (8.84). The concentrations of Cd measured in Atanasovsko Lake in the fall were in the order of 0.0125 µg/L—the highest value recorded for all the studied water bodies. The concentrations of zinc and nickel in the waters did not exceed the norms in Bulgarian legislation. The dynamics of biogenic elements (Zn and Ni) in crustaceans were inversely proportional to those found in the waters. The levels of the toxicant cadmium as well as the metals zinc and nickel in the species Gammarus spp. and Atremia spp., inhabiting all analyzed water bodies, were significantly lower than those specified in the Bulgarian and European legislation. The pH and electrical conductivity parameters of the tested waters, as well as the concentrations of the heavy metals, cadmium, zinc and nickel, were within the recommended values. High positive correlations were determined between the content of nickel, on the one hand, and cadmium and zinc, on the other, in the organism of the investigated crustaceans. A longer period of research is needed to accurately determine the degree of contamination of these waters.

Keywords

• pollution
• lead
• zinc
• nickel
• accumulation
• crustaceans