Nature Communications (Jan 2021)
p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response
- Shun Kageyama,
- Sigurdur Runar Gudmundsson,
- Yu-Shin Sou,
- Yoshinobu Ichimura,
- Naoki Tamura,
- Saiko Kazuno,
- Takashi Ueno,
- Yoshiki Miura,
- Daisuke Noshiro,
- Manabu Abe,
- Tsunehiro Mizushima,
- Nobuaki Miura,
- Shujiro Okuda,
- Hozumi Motohashi,
- Jin-A Lee,
- Kenji Sakimura,
- Tomoyuki Ohe,
- Nobuo N. Noda,
- Satoshi Waguri,
- Eeva-Liisa Eskelinen,
- Masaaki Komatsu
Affiliations
- Shun Kageyama
- Department of Physiology, Juntendo University Graduate School of Medicine
- Sigurdur Runar Gudmundsson
- Molecular and Integrative Biosciences Research Programme, University of Helsinki
- Yu-Shin Sou
- Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine
- Yoshinobu Ichimura
- Department of Physiology, Juntendo University Graduate School of Medicine
- Naoki Tamura
- Department of Anatomy and Histology, Fukushima Medical University School of Medicine
- Saiko Kazuno
- Laboratory of Proteomics and Biomolecular Science, Research Support Center, Juntendo University Graduate School of Medicine
- Takashi Ueno
- Laboratory of Proteomics and Biomolecular Science, Research Support Center, Juntendo University Graduate School of Medicine
- Yoshiki Miura
- Laboratory of Proteomics and Biomolecular Science, Research Support Center, Juntendo University Graduate School of Medicine
- Daisuke Noshiro
- Institute of Microbial Chemistry (BIKAKEN)
- Manabu Abe
- Department of Animal Model Development, Brain Research Institute, Niigata University
- Tsunehiro Mizushima
- Picobiology Institute, Graduate School of Life Science, University of Hyogo, 3-2-1, Kouto, Kamigori-cho
- Nobuaki Miura
- Bioinformatics Laboratory, Niigata University Graduate School of Medical and Dental Sciences
- Shujiro Okuda
- Bioinformatics Laboratory, Niigata University Graduate School of Medical and Dental Sciences
- Hozumi Motohashi
- Department of Gene Expression Regulation, Institute of Development, Aging and Cancer, Tohoku University
- Jin-A Lee
- Department of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University
- Kenji Sakimura
- Department of Animal Model Development, Brain Research Institute, Niigata University
- Tomoyuki Ohe
- Department of Pharmaceutical Sciences, Faculty of Pharmacy, Keio University
- Nobuo N. Noda
- Institute of Microbial Chemistry (BIKAKEN)
- Satoshi Waguri
- Department of Anatomy and Histology, Fukushima Medical University School of Medicine
- Eeva-Liisa Eskelinen
- Molecular and Integrative Biosciences Research Programme, University of Helsinki
- Masaaki Komatsu
- Department of Physiology, Juntendo University Graduate School of Medicine
- DOI
- https://doi.org/10.1038/s41467-020-20185-1
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
Liquid-liquid phase separation of p62/SQSTM1 has been previously described, although the significance in vivo remains unclear. Here the authors show p62 droplets contain ubiquitin, autophagy-related proteins and Keap1 to serve as platform of not only autophagosome formation but also Nrf2 activation.
