Nature Communications (Dec 2022)
An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing
- Feng Xiong,
- Hong Yang,
- Yi-Ge Song,
- Hai-Bin Qin,
- Qing-Yang Zhang,
- Xian Huang,
- Wei Jing,
- Manfei Deng,
- Yang Liu,
- Zhixiang Liu,
- Yin Shen,
- Yunyun Han,
- Youming Lu,
- Xiangmin Xu,
- Todd C. Holmes,
- Minmin Luo,
- Fei Zhao,
- Min-Hua Luo,
- Wen-Bo Zeng
Affiliations
- Feng Xiong
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- Hong Yang
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- Yi-Ge Song
- Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology
- Hai-Bin Qin
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- Qing-Yang Zhang
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- Xian Huang
- Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology
- Wei Jing
- Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology
- Manfei Deng
- Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology
- Yang Liu
- National Institute of Biological Sciences
- Zhixiang Liu
- National Institute of Biological Sciences
- Yin Shen
- Eye Center, Renmin Hospital, Wuhan University
- Yunyun Han
- Eye Center, Renmin Hospital, Wuhan University
- Youming Lu
- Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology
- Xiangmin Xu
- Department of Anatomy and Neurobiology, School of Medicine, University of California
- Todd C. Holmes
- Center for Neural Circuit Mapping, School of Medicine, University of California
- Minmin Luo
- National Institute of Biological Sciences
- Fei Zhao
- Chinese Institute for Brain Research
- Min-Hua Luo
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- Wen-Bo Zeng
- State Key Laboratory of Virology, CAS Center for Excellence in Brain Science and Intelligence Technology, Wuhan Institute of Virology, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-022-35355-6
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
Minimizing toxicity of herpes simplex virus 1 (HSV) based tools for anterograde tracing is important for functional studies. Here the authors generate an anterograde monosynaptic tracer system based on HSV amplicon, which shows fast tracing, bright labeling, low toxicity, input-defined postsynaptic neurons’ anterograde monosynaptic tracing feature, and has potential for functional mapping.
