Nature Communications (Sep 2018)

Identifying weak interdomain interactions that stabilize the supertertiary structure of the N-terminal tandem PDZ domains of PSD-95

  • Inna S. Yanez Orozco,
  • Frank A. Mindlin,
  • Junyan Ma,
  • Bo Wang,
  • Brie Levesque,
  • Matheu Spencer,
  • Soheila Rezaei Adariani,
  • George Hamilton,
  • Feng Ding,
  • Mark E. Bowen,
  • Hugo Sanabria

DOI
https://doi.org/10.1038/s41467-018-06133-0
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Biologically relevant weak and transient interdomain interactions within proteins are difficult to analyze. Here, the authors combine multiscale molecular dynamics simulations and high-precision FRET experiments to characterize interactions between the tandem PDZ domains of PSD-95, revealing previously hidden conformational states.