New Type of Tannins Identified from the Seeds of <i>Cornus officinalis</i> Sieb. et Zucc. by HPLC-ESI-MS/MS
Jun Li,
Lin Chen,
Hua Jiang,
Min Li,
Lu Wang,
Jia-Xing Li,
Yue-Yue Wang,
Qing-Xia Guo
Affiliations
Jun Li
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Lin Chen
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Hua Jiang
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Min Li
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Lu Wang
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Jia-Xing Li
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Yue-Yue Wang
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
Qing-Xia Guo
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471023, China
There is a lack of information on the compound profile of Cornus officinalis Sieb. et Zucc. seeds. This greatly affects their optimal utilization. In our preliminary study, we found that the extract of the seeds displayed a strong positive reaction to the FeCl3 solution, indicating the presence of polyphenols. However, to date, only nine polyphenols have been isolated. In this study, HPLC-ESI-MS/MS was employed to fully reveal the polyphenol profile of the seed extracts. A total of 90 polyphenols were identified. They were classified into nine brevifolincarboxyl tannins and their derivatives, 34 ellagitannins, 21 gallotannins, and 26 phenolic acids and their derivatives. Most of these were first identified from the seeds of C. officinalis. More importantly, five new types of tannins were reported for the first time: brevifolincarboxyl-trigalloyl-hexoside, digalloyl-dehydrohexahydroxydiphenoyl (DHHDP)-hexdside, galloyl-DHHDP-hexoside, DHHDP-hexahydroxydiphenoyl(HHDP)-galloyl-gluconic acid, and peroxide product of DHHDP-trigalloylhexoside. Moreover, the total phenolic content was as high as 79,157 ± 563 mg gallic acid equivalent per 100 g in the seeds extract. The results of this study not only enrich the structure database of tannins, but also provide invaluable aid to its further utilization in industries.
