Frontiers in Immunology (Jan 2022)
l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
- Xiangbing Mao,
- Xiangbing Mao,
- Xiangbing Mao,
- Xiangbing Mao,
- Rui Sun,
- Rui Sun,
- Rui Sun,
- Rui Sun,
- Qingxiang Wang,
- Qingxiang Wang,
- Qingxiang Wang,
- Qingxiang Wang,
- Daiwen Chen,
- Daiwen Chen,
- Daiwen Chen,
- Daiwen Chen,
- Bing Yu,
- Bing Yu,
- Bing Yu,
- Bing Yu,
- Jun He,
- Jun He,
- Jun He,
- Jun He,
- Jie Yu,
- Jie Yu,
- Jie Yu,
- Jie Yu,
- Junqiu Luo,
- Junqiu Luo,
- Junqiu Luo,
- Junqiu Luo,
- Yuheng Luo,
- Yuheng Luo,
- Yuheng Luo,
- Yuheng Luo,
- Hui Yan,
- Hui Yan,
- Hui Yan,
- Hui Yan,
- Jianping Wang,
- Jianping Wang,
- Jianping Wang,
- Jianping Wang,
- Huifen Wang,
- Huifen Wang,
- Huifen Wang,
- Huifen Wang,
- Quyuan Wang,
- Quyuan Wang,
- Quyuan Wang,
- Quyuan Wang
Affiliations
- Xiangbing Mao
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Xiangbing Mao
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Xiangbing Mao
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Xiangbing Mao
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Rui Sun
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Rui Sun
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Rui Sun
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Rui Sun
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Qingxiang Wang
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Qingxiang Wang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Qingxiang Wang
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Qingxiang Wang
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Daiwen Chen
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Daiwen Chen
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Daiwen Chen
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Daiwen Chen
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Bing Yu
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Bing Yu
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Bing Yu
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Bing Yu
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Jun He
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Jun He
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Jun He
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Jun He
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Jie Yu
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Jie Yu
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Jie Yu
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Jie Yu
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Junqiu Luo
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Junqiu Luo
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Junqiu Luo
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Junqiu Luo
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Yuheng Luo
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Yuheng Luo
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Yuheng Luo
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Yuheng Luo
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Hui Yan
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Hui Yan
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Hui Yan
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Hui Yan
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Jianping Wang
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Jianping Wang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Jianping Wang
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Jianping Wang
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Huifen Wang
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Huifen Wang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Huifen Wang
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Huifen Wang
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- Quyuan Wang
- Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China
- Quyuan Wang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, China
- Quyuan Wang
- Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Chengdu, China
- Quyuan Wang
- Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Chengdu, China
- DOI
- https://doi.org/10.3389/fimmu.2021.817583
- Journal volume & issue
-
Vol. 12
Abstract
Inflammatory bowel disease (namely, colitis) severely impairs human health. Isoleucine is reported to regulate immune function (such as the production of immunoreactive substances). The aim of this study was to investigate whether l-isoleucine administration might alleviate dextran sulfate sodium (DSS)-induced colitis in rats. In the in vitro trial, IEC-18 cells were treated by 4 mmol/L l-isoleucine for 12 h, which relieved the decrease of cell viability that was induced by TNF-α (10 ng/ml) challenge for 24 h (P <0.05). Then, in the in vivo experiment, a total of 44 Wistar rats were allotted into 2 groups that were fed l-isoleucine-supplemented diet and control diet for 35 d. From 15 to 35 d, half of the rats in the 2 groups drank the 4% DSS-adding water. Average daily gain, average daily feed intake and feed conversion of rats were impaired by DSS challenge (P <0.05). Drinking the DSS-supplementing water also increased disease activity index (DAI) and serum urea nitrogen level (P <0.05), shortened colonic length (P <0.05), impaired colonic enterocyte apoptosis, cell cycle, and the ZO-1 mRNA expression (P <0.05), increased the ratio of CD11c-, CD64-, and CD169-positive cells in colon (P <0.05), and induced extensive ulcer, infiltration of inflammatory cells, and collagenous fiber hyperplasia in colon. However, dietary l-isoleucine supplementation attenuated the negative effect of DSS challenge on growth performance (P <0.05), DAI (P <0.05), colonic length and enterocyte apoptosis (P <0.05), and dysfunction of colonic histology, and downregulated the ratio of CD11c-, CD64-, and CD169-positive cells, pro-inflammation cytokines and the mRNA expression of TLR4, MyD88, and NF-κB in the colon of rats (P <0.05). These results suggest that supplementing l-isoleucine in diet improved the DSS-induced growth stunting and colonic damage in rats, which could be associated with the downregulation of inflammation via regulating TLR4/MyD88/NF-κB pathway in colon.
Keywords