Nature Communications (Feb 2019)

Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis

  • Wei Chen,
  • Jun Yao,
  • Jie Meng,
  • Wenjing Han,
  • Yong Tao,
  • Yihua Chen,
  • Yixin Guo,
  • Guizhi Shi,
  • Yang He,
  • Jian-Ming Jin,
  • Shuang-Yan Tang

DOI
https://doi.org/10.1038/s41467-019-08781-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

Read online

Metabolic engineering usually focuses on manipulating enzyme(s) within a single pathway. Here, the authors show that a promiscuous enzymatic activity-based multiple-pathway design can minimize cell metabolic burden and allow carbon flow rearrangement, leading to efficient hydroxytyrosol biosynthesis.