PRODUCTION OF REDUCING SUGARS AND CELLULASE BY LOCAL STRAIN OF Trichoderma viride FROM TOMATO FRUITS WASTES USING SOLID STATE FERMENTATION

Abstract

A Local strain of Trichoderma viride was isolated from the soil. The effect of incubation period and cutlure components of reducing sugars and cellulase were studied. Resutls showed that the optimum incubition period for highest production of reducing sugar and cellulase was 6 days. Ammonium phosphate at 0.06% concentration as the nitrogen source gave the maximum reducing sugar (67.88) g/kg and enzyme activit 2.68) and 2.78 unit /g) by using filter papar F.pase and carboxy methyl cellulose(CMC) respectively. The study also showed that addition of 0.08 % KH2PO4 had positive effect on the production of reducing sugar which gave 69.76) g/l) and highest enzyme activity 2.82) and 2.73 unit /g) .Magnesium sulfate at 0.06% concentration the highest enzyme activity by using Fpase and CMC were optioned 2.74 ) and 2.58 unit /g) respectively and 64.46 g/kg reduc

Keywords

• cmc
• pase
• cellulose
• trichoderma viride
• reducing sugars
• fpase