Hypouricemic Effects of Extracts From Agrocybe aegerita on Hyperuricemia Mice and Virtual Prediction of Bioactives by Molecular Docking

Tianqiao Yong; Tianqiao Yong; Shaodan Chen; Shaodan Chen; Yizhen Xie; Yizhen Xie; Ou Shuai; Ou Shuai; Xiangmin Li; Xiangmin Li; Diling Chen; Diling Chen; Jiyan Su; Jiyan Su; Chunwei Jiao; Chunwei Jiao; Yalei Liang; Yalei Liang

doi:10.3389/fphar.2018.00498

Frontiers in Pharmacology (May 2018)

Hypouricemic Effects of Extracts From Agrocybe aegerita on Hyperuricemia Mice and Virtual Prediction of Bioactives by Molecular Docking

Tianqiao Yong,
Tianqiao Yong,
Shaodan Chen,
Shaodan Chen,
Yizhen Xie,
Yizhen Xie,
Ou Shuai,
Ou Shuai,
Xiangmin Li,
Xiangmin Li,
Diling Chen,
Diling Chen,
Jiyan Su,
Jiyan Su,
Chunwei Jiao,
Chunwei Jiao,
Yalei Liang,
Yalei Liang

Affiliations

Tianqiao Yong: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Tianqiao Yong: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Shaodan Chen: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Shaodan Chen: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Yizhen Xie: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Yizhen Xie: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Ou Shuai: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Ou Shuai: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Xiangmin Li: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Xiangmin Li: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Diling Chen: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Diling Chen: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Jiyan Su: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Jiyan Su: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Chunwei Jiao: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Chunwei Jiao: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China
Yalei Liang: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
Yalei Liang: Guangdong Yuewei Edible Fungi Technology Co., Guangzhou, China

DOI: https://doi.org/10.3389/fphar.2018.00498
Journal volume & issue: Vol. 9

Abstract

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Agrocybe aegerita has long been utilized for promoting diuresis in traditional Chinese medicine (TCM) with a close correlation to hypouricemia. Ethanol (AAE) and water (AAW) extracts of the compound led to a remarkable decrease in serum uric acid levels (SUA) in hyperuricemia mice, approaching that of the normal control. Both AAE and AAW exhibited suppression effects on hepatic xanthine oxidase (XOD) activities and elevation effects on renal OAT1 (organic anion transporter 1). However, only little negative impact was observed on the inner organ functions. The molecular docking was used to screen our in-home compound database for A. aegerita, and four compounds including 2-formyl-3,5-dihydroxybenzyl acetate, 2,4-dihydroxy-6-methylbenzaldehyde, 2-(6-hydroxy-1H-indol-3-yl)acetamide, and 6-hydroxy-1H-indole-3-carbaldehyde (HHC) were identified as potential active compounds. Their inhibitory mechanism on XOD might be attributed to their localization in the tunnel for the entrance of substrates to XOD active site, preventing the entrance of the substrates. To confirm the activity of the screened compounds experimentally, HHC was selected due to its high ranking and availability. The assaying result suggested the significant inhibitory activity of HHC on XOD. Also, these compounds were predicted to carry good ADME (absorption, distribution, metabolism, and excretion) properties, thereby necessitating further investigation. The current results provided an insight into the hypouricemic effects of macrofungi and their bioactives, which might provide the significant theoretical foundation for identifying and designing novel hypouricemia compounds.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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