Establishing the Initial Stage of Psoriasis in Cell Culture

Abstract

Psoriasis is a chronic and relapsing skin disease that affects approximately 2⁻3% of the world population and is considered as an inflammatory disease. Defective keratinocyte proliferation and differentiation programs, aberrant immune responses are the major factors that have been implicated in its pathogenesis. Thick scaly plaques, which are psoriasis characteristic formed by the abnormal maturation of keratinocytes and epidermal hyperplasia. The aim of this study is to establish the initial stage of psoriasis model by LPS (lipopolysaccharide) treatment to HaCaT (transformed keratinocyte) cells. MTT assay of LPS was performed to hallmark the initial stage of psoriasis. ELISA assay was performed to determine the cytokine release as response to LPS for psoriasis initial stage. According to the MTT assay results 200 ng/mL used as the test dose and 8µg/mL LPS determined as the toxic dose at 72th hour. LL-37 was the first cytokine whose expression increased in the formation of the immune response. Besides, TNF-α release occurs soon after. The presence of immune system cells is needed to maintain IL-17 and IL-1β release. The cytokine release at the 72th hour decreased to control level due to the lack of immune cells in the culture medium.

Keywords

• psoriasis
• HaCaT
• LPS
• cytokine