Correlation of Early Growth Response 3 and Interleukin 6 Expression Levels with Coronary Heart Disease

Abstract

Background Coronary heart disease (CHD) is the leading cause of death world wide. Coronary angiography is often used as an effective approach for the diagnosis of CHD. However, coronary angiography accounts for relatively low percentagein examination approaches due to the limitation of various conditions in primary care, and more meaningful biomarkers need to be explored to provide a basis for primary care physicians to diagnose and treat CHD. In the previous study of our research group, it is found that early growth response 3 (Egr3) gene may be a susceptibility factor in the heterogeneity of CHD pathogenesis, and there are few reports on the correlation between CHD and Egr3 gene and inflammatory biomarkers. Objective To investigate the correlation between Egr3, interleukin-6 (IL-6) and CHD, as well as their expression levels and the severity of coronary stenosis, so as to provide a favorable laboratory basis for clinical diagnosis and treatment inprimary care. Methods A total of 110 patients who admitted to the Fifth Affiliated Hospital of Xinjiang Medical University for CHD symptoms and completed coronary artery angiography (CAG) during June to December 2021 were collected and divided into the mild stenosis group (group A≤52 points, n=50) , moderate-severe stenosis group (group B>52 points, n=30) and control group (group C, n=30) according to the CAG results and median Gensini score (52 points) . Serum Egr3 and IL-6 levels of the included patients were measured by enzyme-linked immunosorbent assay. Results The IL-6 level in group B was higher than that in group A and group C (P<0.05) , and Egr3 expression level in the group A and group B was higher than that in group C, respectively (P<0.05) ; the area under the ROC curve of Egr3 expression level in the diagnosis of coronary heart disease was 0.648, with a sensitivity of 35.0% and specificity of 93.3%. There was positive correlation between IL-6 and Egr3 in CHD patients (r=0.231, P<0.01) ; Egr3 and IL-6 levels were positively correlated with Gensini score (rs=0.39, 0.317, P<0.01) . Conclusions Egr3 has good specificity for the diagnosis of CHD, and the expression levels of Egr3 and IL-6 were positively correlated with the severity of coronary stenosis.

Keywords

• coronary disease|early growth response 3|intercellular signaling peptides and proteins|interleukin-6|gensini score|coronary stenosis|correlation analysis