Biotropia: The Southeast Asian Journal of Tropical Biology (Jan 2005)
PRODUCTION AND UTILIZATION OF CELLULASE FROMTRICHODERMA VIRIDE
Abstract
An appropriate preservation technology for forage such as silage n eeds to be developed in order to overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by decreasing the fibre contents (Neutral Detergent Fibr e, Acid Detergent Fibre and lignin). The research was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride and 2). Comparative test using crude enzymes from Trichoderma viride strain QM 9414 (CETV) and commercial cellulase (Cellulase “Onozuka R-10”, heidelberg) on rice straw silage. The treatments were arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1, P2= Treated with CETV at 3.8 IU kg-1, P3=Treated with CETV at 7.6 IU kg-1 and P4= Treated with CETV at 11.4 IU kg-1 of fresh rice straw. All treatments were enriched with 5% molasses. The result of the research indicated that: 1). Th e activity of crude enzymes from Trichoderma viride strain QM 9414 (CETV) was 1.52 IUml-1. 2). The addition of both enzymes did not decrease dry matter of rice straw silage; however, organic matter significantly decreased (p<0.05) by addition of commercial cellulase. 3). The addition of both enzymes decreased fibre c ontents (NDF), furthermore increased (p<0.05) in sacco digestibility of dry matter and organic matter of rice straw silage.
